Fig. 4

TMEM52B ECD-derived peptides suppress cancer cell invasion in an EGFR-dependent manner. a Cells were treated with EGF (10 ng/mL) and the peptides for 48 h prior to lysis and immunoblotting. Densitometric quantification was performed. Phosphorylated proteins were normalized against the amount of corresponding total protein. b Cells were allowed to invade Matrigel for 48 h in the absence or presence of EGF (10 ng/mL) and the peptides. Cell invasion was determined by calculating the cell-stained area relative to the total area using ImageJ software. c HCT-15 cells were transfected with siRNA specific to EGFR for 48 h. Transfected cells were subjected to invasion assay and lysis prior to immunoblot analysis. Values represent mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001, compared with siControl + Vehicle (C). n.s., non-significant