Fig. 5

LST treatment facilitated the induction of mitophagy in the kidney tubule of DKD mice. A: Immunofluorescence microscopy showed LC3 (green) and VDAC1 (red) co-localization in renal tubule of CON subset (top panel). LC3 fluorescence intensities diminished in the DKD subset but was restored by LST treatment. B and C: Co-localization fluorescence intensities and analyses of morphometry validated the findings. D: Western blot analysis of LC3II/I, Atg5, SQSTM1, TOMM20, Beclin, and VDAC1 protein expression. The result is shown as mean ± SE, *P < 0.05, vs. CON subset; # P < 0.05, vs. DKD subset. n = 8