Fig. 2

The degradation of XPO1 protein exhibits a positive correlation with the sensitivity of MM cells to SEL treatment. A, B and C Cell viability of MM cell lines treated with increasing concentrations of drugs for 48 h, and the IC50 of SEL (A), ELT (B), VER (C) for each cell line. D Cell apoptosis rates of different MM cell lines after treated with indicated concentrations of SEL for 48 h. E Representative immunoblotting images and quantitative analysis of XPO1 protein levels in different MM cell lines treated with increasing concentrations of SEL for 24 h. F and G Immunofluorescent staining of RanBP1(green) in NCI-H929 (F) and MM.1S (G) cells after treated with DMSO, MG132 (10 μM) and /or SEL (500 nM) for 12 h. Nucleus were stained with DAPI (blue). The data are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001