Fig. 3
From: Cancer-associated fibroblasts induce almonertinib resistance in non-small cell lung cancer
CAFs alleviated the inhibitory effects of almonertinib on NSCLC cells. The supernatants of NFs and CAFs were centrifuged at 1000xg for 15 min and collected as conditioned medium (CM). H1975 and A549 cells were treated with different CMs for 48 h. Then, they were exposed to 2 or 4 µM almonertinib. (A-B) Colony formation was evaluated via a colony formation assay. (C-D) Cell migration was determined by a Transwell migration assay (magnification, ×100). (E-F) Cell invasion was measured via a Transwell invasion assay (magnification, ×100). The values are the means ± SEMs. Significance: **p < 0.01, ***p < 0.001, ****p < 0.0001