Fig. 2

CAFs were successfully induced by TGF-β1, and almonertinib increased the TGF-β1 secretion of H1975 cells. HFL1 cells were stimulated with 10 ng/mL TGF-β1 for 24–48 h, after which they were passaged 2–3 times. (A) Morphology was observed under a microscope (magnification, ×40). (B) The expression levels of α-SMA and FAP in the NF, CAF-24 h and CAF-48 h groups were detected by Western blotting. (C) The secretion of TGF-β1 in H1975 cells treated with 0, 2, or 4 µM almonertinib was determined by ELISA. The values are the means ± SEMs. Significance: ns, not significant; **p < 0.01; ***p < 0.001