Fig. 8

miR-223-3p targeted IKKα mRNA and inhibited the NF-κB signaling pathway. (A) The level of miR-223-3p in HK2 cells. Western blot images and quantitative band density analyses of IKKα (B-C), NF-κBp65 and p-NF-κBp65 (D-F), P-IKBα and IKBα (G-I), NLRP3 and Caspase-1 (J-L) in different groups. (M) Immunofluorescence staining of NF-κB p65 in HK2 cells (at 400×magnification). (N) The binding site of miR-223-3p and CHUK predicted by TargetScan. (O) Relative luciferase activity. **P < 0.05 vs. Group (TGF-β1+, miR-223-3p-, CHUK plasmid-). **** P < 0.01 vs. Group (TGF-β1+, miR-223-3p-, CHUK plasmid-)