Fig. 6

miR-223-3p inhibited EMT of HK2 cells. (A) Cell activity. (B) The expression of miR-223-3p. (C) The expression of IKKα mRNA. (D) Transfection efficiency of miR-223-3p. (E) Transfection efficiency of CHUK. (F-G) Western blot images and quantitative band density analyses of α-SMA in HK2 cells. (H-I) mRNA expression of α-SMA and Col-1a in HK2 cells. (J) Immunofluorescence staining of α-SMA in the HK2 cells (at 400×magnification). **P < 0.05 vs. Group (TGF-β1+, miR-223-3p-, CHUK plasmid-). **** P < 0.01 vs. Group (TGF-β1+, miR-223-3p-, CHUK plasmid-)