Fig. 6

Circ-ITCH promotes the ubiquitination degradation of HOXC10 through enhancing BRCA1 mRNA stability. (A) The associations among circ-ITCH, IGF2BP2 protein and BRCA1 mRNA were predicted by ENCORI database. (B) The co-localization of circ-ITCH and IGF2BP2 protein in BM-MSCs was detected by FISH combined with immunofluorescence staining. Scale bar, 50 μm. Green, circ-ITCH. Red, IGF2BP2. Blue, Hoechest. (C) BM-MSCs were overexpressed circ-ITCH, and the interaction between circ-ITCH and IGF2BP2 was detected by RNA pull-down assay. Anti-sense probes acted as a negative control. (D) The associations among circ-ITCH, IGF2BP2 protein and BRCA1 mRNA were validated by RIP assay. Normal IgG served as a negative control. (E) The interaction between IGF2BP2 protein and BRCA1 mRNA was detected by RIP assay under circ-ITCH overexpression. Normal IgG served as a negative control. (F) BM-MSCs were silenced IGF2BP2, and the mRNA level of BRCA1 was detected by qRT-PCR. **P < 0.01 and ***P < 0.001