Fig. 3

Circ-ITCH promotes osteogenic differentiation of BM-MSCs under microgravity simulation. Human BM-MSCs were overexpressed circ-ITCH and transfected BM-MSCs were cultured in osteogenic differentiation medium under microgravity simulation for 14 days. (A) The expression level of circ-ITCH was detected by qRT-PCR. (B) The mineralization process in BM-MSCs was monitored by ARS staining. (C) The ALP activity in BM-MSCs was detected using commercial kit. (D) The mRNA levels of ALP, OPN, OCN and HOXC10 in BM-MSCs were analysed by qRT-PCR. (E) The protein levels of ALP, OPN, OCN and HOXC10 in BM-MSCs were detected by western blot. *P < 0.05, **P < 0.01, and ***P < 0.001