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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Circ-ITCH promotes the ubiquitination degradation of HOXC10 to facilitate osteogenic differentiation in disuse osteoporosis through stabilizing BRCA1 mRNA via IGF2BP2-mediated m6A modification

Fig. 2

Circ-ITCH is decreased in BM-MSCs under microgravity simulation. Human BM-MSCs were cultured in osteogenic differentiation medium under microgravity simulation for 7 or 14 days. (A) The mineralization process in BM-MSCs was monitored by ARS staining. (B) The mRNA levels of osteogenic differentiation markers ALP, OPN and OCN in BM-MSCs were detected by qRT-PCR. (C) The protein levels of osteogenic differentiation markers ALP, OPN and OCN in BM-MSCs were measured by western blot. (D) The ALP activity in BM-MSCs was analysed using commercial kit. (E) The expression level of circ-ITCH in BM-MSCs was detected by qRT-PCR. *P < 0.05 and **P < 0.01

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Journal of Translational Medicine

ISSN: 1479-5876

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