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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Circ-ITCH promotes the ubiquitination degradation of HOXC10 to facilitate osteogenic differentiation in disuse osteoporosis through stabilizing BRCA1 mRNA via IGF2BP2-mediated m6A modification

Fig. 1

Circ-ITCH is downregulated in rat BM-MSCs derived from DOP model. Rats were randomly divided into two groups: sham and DOP groups (n = 8 per group). (A) The BMD of rats was detected by DXA. (B) The bone parameters including Tb.Th, Tb.N and Tb.Sp were examined by micro-CT. (C) The histological changes of bones were assessed by H&E and Alcian blue stainings. Scale bar, 100 μm. (D) The mRNA levels of osteogenic differentiation markers ALP, OPN and OCN in rat BM-MSCs were detected by qRT-PCR. (E) The protein levels of osteogenic differentiation markers ALP, OPN and OCN in rat BM-MSCs were measured by western blot. (F) The expression level of circ-ITCH in rat BM-MSCs was tested by qRT-PCR. *P < 0.05, **P < 0.01, and ***P < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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