Fig. 4

Cellular internalization of hEnSC-derived small EVs by PC12 cells. a PC12 cells were incubated with different dil dye labeled small EVs concentration (0, 50, 100, and 150 µg/ml) for 12 h, and cells nuclei were stained with DAPI. Increased dye labeled small EVs were observed inside the cells by increasing small EVs concentration. Also as shown in figure stained small EVs (red spots) was completely penetrate in cells and concentrated around the nuclei (blue core) (scale bar = 100 μm); b Also, the intensity of Dil stain was measured by image j. ns not significant/****P < 0.0001. Each experiment was investigated in triplicate (n = 3)