Fig. 3

Characterization of hEnSC-derived small EVs. a Illustration of size distribution of the isolated hEnSC-derived small EVs by SEM (scale bar 1 μm); b TEM photomicrographs of hEnSC-derived small EVs (scale bar = 100 nm); c Size distribution of hEnSC-derived small EVs by DLS; d Determination of small EVs concentration based on the mass concentration of BSA (small EVs concentration and 1000 µg/ml concentration of BSA was no significant difference). e The expression of small EVs marker proteins CD9, CD63, CD81 and cytochrome C (as a negative marker) by western blot assay. Each experiment was investigated in triplicate (n = 3)