Table 1 Primer design sequence
Target gene | Sequence(5’-3’) |
|---|---|
β-actin | F: GATCAGCAAGCAGGAGTACGA |
R: GGGTGTAAAACGCAGCTCA | |
Piezo1 | F: CTTCGGGTTGGAGAGGTACG |
R: ACTCAAAGGCTCTTCGGCTC | |
GPX4 | F: GCCTCGCAATGAGGCAAAAC |
R: CAAACTGGTTGCAGGGGAAG | |
COX2 | F: CAGGACTCTGCTCACGAAGG |
R: ATCCAGTCCGGGTACAGTCA | |
FTH1 | F: AAGTGCGCCAGAACTACCAC |
R: AGCCACATCATCTCGGTCAA | |
TOM20 | F: GTGCGGTGTGTTGTCTGTTG |
R: GATGGAACACCCCAGAGACG | |
SOD | F: TTCTCGTCTTGCTCTCTCTGG |
R: CTTCTGCTCGAAGTGGATGGT |