Fig. 1

GA alleviates skeletal muscle injury and ferroptosis caused by excessive exercise in mice A Images of mouse skeletal muscle stained with H&E and Sirius red, “_” represents 100 μm B-C Detection of IL-6, TNF-α, CK, LDH, Fe²⁺ and MDA levels in skeletal muscle tissue by ELISA D-E Western blot bands represent the expression levels of Piezo1, GPX4, FTH1 and COX2 proteins in different groups of mouse tissues, with histograms representing the quantitative data of each index standardized to β-actin F Detection of Piezo1, GPX4, FTH1 and COX2 mRNA levels in skeletal muscle tissue by RT-qPCR G-H Immunohistochemical detection of Piezo1 and GPX4 expression in skeletal muscle tissue of different groups of mice, with “_” indicating 100 μm, accompanied by quantitative histograms Values are expressed as the mean ± SD from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001