Fig. 1

Construction of single-cell transcriptome profiles. A T-SNE plot depicting the distribution of 15,126 tumor cells from 5 RCC patients. B T-SNE plot showing the annotated single-cell profiles consisting of 12 cell types. C Bubble plots showing marker genes expressed in the major cell types; the color of the dots reflects the level of expression, and the size of the dots represents the percentage of cells expressing the marker genes in the different cell types. D Table showing the number of different cells in each group and the percentage of the cells in each group. E T-SNE plots demonstrating the distribution of the expression of PDCD1 and its receptor genes. F Bubble plots showing the distribution of the expression of the immune checkpoint LAG3 and its receptor genes in different subgroups. Pri.R, primary resistance group. Sen, sensitive group. Pcc, peritubular capillary cells