Fig. 5

Pharmacological doses of Vit-C induce apoptosis in MDA-MB-231 and MCF-7 spheroids. A and B Flow cytometry analysis by using Annexin V-FITC/PI staining to measure apoptosis in MDA-MB-231 and MCF-7 spheroids after treatment with five pharmacological doses of Vit-C (1, 5, 10, 15, and 20 mM) for 72 h, respectively. C and B Caspase-3/7 activity measurement by Caspase-Glo® 3/7 3D Assay in MDA-MB-231 and MCF-7 spheroids subjected to treatment with five pharmacological concentrations of Vit-C (1, 5, 10, 15, and 20 mM) for 72 h, respectively. The levels of the statistical significance were determined as follows: **P < 0.01, ***P < 0.001, ****P < 0.0001, and *P < 0.05, ns, not significant. Two-tailed unpaired student’s t-test was employed in both cases, and the error bars represent the mean ± SEM. The data are representative of three independent experiments (n = 3)