Fig. 1
Characterization of MDA-MB-231 and MCF-7 Tumor spheroids. A Schematic diagram showing the protocol utilized to develop MDA-MB-231 and MCF-7 tumor spheroids. B Representative brightfield images for the growth kinetics of the MDA-MB-231 and MCF-7 tumor spheroids. Cells at the density of 2 × 104 cells/well were seeded in a 96-well agarose-coated plate and incubated over a period of 15 days. Images were acquired starting from day 3 from seeding to day 15. Scale bars = 500 μM; all images are of the same resolution. C A graph showing the growth kinetics of MDA-MB-231 and MCF-7 tumor spheroids, where the diameter of MDA-MB-231 tumor spheroids decreased with time, as compared to MCF-7 tumor spheroids, for which the diameter increased with time