Fig. 6

Spatial and functional characteristics of different metabolic tumor regions in CSCC. (A) Correlation analysis between the expression levels of APP in RNA-seq data of TCGA CSCC tissues and the expression levels of the transcription factor TRPS1 and genes related to tumor metabolism (ITGA2 and PRKCE). (B) Bar graph showing siRNA-mediated knockdown of APP (left) and TRPS1 (right) RNA expression in HeLa cells. (C) Bar graph showing TRPS1 RNA expression in control and knockdown of APP HeLa cells (left) and APP RNA expression in control and knockdown of TRPS1 HeLa cells. (D) Bar graph showing the RNA expression of tumor metabolism-related genes (ITGA2 and PRKCE) in control and knockdown of APP HeLa cells. (E) Cell proliferation measured by CCK8 assay in HeLa cells transfected with si-APP, si-TRPS1, or si-NC. (F-G) The effect of APP or TRPS1 knockdown on HeLa cells’ migration (F) and invasion (G) determined by transwell assays. (H-I) Estimation of the survival probability of CSCC patients based on APP (H) or TRPS1 (I) gene expression levels. The analysis was performed using TCGA’s dataset of 255 CSCC samples. (J) Schematic summary illustrating immune characteristics and associations of APP and TRPS1 with CSCC progression, generated using BioRender (https://biorender.com/). Significant differences were determined using the two-tailed unpaired Student’s t-test (****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant). P values < 0.05 were considered statistically significant