Fig. 5

Spatial and functional characteristics of different metabolic tumor regions in CSCC. (A) Spatial distribution of cancer cells (red dashed line indicates hypermetabolic region, green dashed line indicates hypometabolic region) (left), and other cell types in the different regions of the leading edge and tumor core (middle). Proportion of each cell type in each region (right). (B) Heatmap of cell communication analysis between cancer cells and other cell types. Dot size reflects the significance level of cell communication, and color indicates the average expression level of interacting molecules between cancer cell clusters and clusters of other cell types. (C) Correlation analysis between the expression level of APP and the expression level of genes related to tumor metabolism (ITGA2 and PRKCE) in different cell types. (D) Correlation analysis between the expression level of APP and the tumor metabolic score in different gridded regions. (E) Correlation analysis between the expression level of APP and the expression level of TRPS1 in different cell types. (F-G) Spatial distribution of gene expression of APP (F), PRKCE and ITGA2 (G) in cancer cells. (H) Immunofluorescence microscopy observation of cervical cancer slices, with DAPI (blue), KRT (purple), CD74 (green), APP (red), and TRPS1 (orange) antibody staining