Fig. 4
Spatial and functional characteristics of different metabolic tumor regions in CSCC. (A) Heatmap displaying the expression profiles of 50 metabolic genes in different tumor regions. Colors represent the expression levels of the corresponding genes; the “blue-white-red” pattern maps to the minimum-average-maximum expression values. (B) Heatmap showing transcription factors and their expression levels in different tumor regions. Color intensity represents z-score values, and the size of the dots indicates the regulon specificity score (RSS) of specific transcription factors in different tumor regions. (C) Spatial expression of the TRPS1 in cancer cells of leading edge and tumor core region. (D) PPI network graph depicting the interaction and enrichment analysis results of TRPS1 target genes. Each pathway is presented as circular nodes in the network graph, with node size proportional to the number of input genes, and node color indicating the cluster it belongs to. Pathways with a similarity score greater than 0.3 are connected by edges, and edge thickness represents the degree of similarity between them. (E) RNA velocity streams of leading edge and tumor core region, colored by cell annotations for leading edge and tumor core regions