Fig. 3

Metabolic score and copy number variation analysis in different tumor regions of CSCC. (A-B) Spatial score of oxidative phosphorylation, hypoxia, glycolysis, lactate metabolism, lipid metabolism, and pentose phosphate pathway in the leading edge (A) and tumor core region (B) of Stereo-seq samples. (C-D) Grid division of the leading edge and tumor core regions, with yellow representing high metabolic scores and blue representing low metabolic scores. Line plot displaying the distribution of Median metabolic pathway scores across the five regions (C). The heatmap illustrating GSVA scores for various metabolic pathways in different grid regions (D). (E) Comparison of copy number variation (CNV) between cancer cells in different regions, including leading edge, hypermetabolic region in the tumor core region, and hypometabolic region in the tumor core region. The CNV status of each cell is displayed for chromosomes 1 to 22