Fig. 1

Spatial expression patterns of cells in the leading edge and tumor core regions of CSCC. (A) Overview of the schematic representation of the study design. Tumor tissues from CSCC patients were processed into single-cell suspensions, and unsorted cells were subjected to scRNA-seq using 10x Genomics. ST was performed on normal tissue and tumor slices using Stereo-seq, generating ST data. Created with BioRender. (B) UMAP plots of cells identified from scRNA-seq data of three CSCC tissues. Different colors represent distinct cell types. (C) The integrated analysis of single-cell transcriptome and spatial transcriptome data using cell2location is depicted. Created with BioRender. (D-F) Cellular spatial distribution and proportions of reads count for each cell type in normal tissue (D), leading edge region (E), and tumor core region (F)