Fig. 6

miR-1-3p directly targets and down-regulates E2F5 expression. The differentially expressed genes in the Exo-miR-1-3p group compared with the Exo-NC group, shown in the volcano plot (A), and the KEGG pathway analysis of differentially expressed genes (B). (C) Predicted target genes of miR-1-3p with TargetScan and miRWalk were cross-analyzed with significantly expression down-regulated genes. (D) E2F5 mRNA level after Exo-miR-1-3p incubation. (E) Putative binding sequence of miR-1-3p to the E2F5 mRNA 3’UTR and results of dual luciferase reporter assays. E2F5 protein expression of KYSE150 and Eca109 cells transfected with miR-1-3p mimics (F) or miR-1-3p inhibitor (G). Immunohistochemical staining (H) and H-scores (I) of E2F5 in mouse lung tissues. *P < 0.05, ***P < 0.001