Fig. 2
From: Advancements in lung regeneration: from bench to bedside
Some strategies for creating airway organoids/spheroids. (a) Spheroids can be produced from different organs like nasal polyps or cells from nasal brushing/curettage. They can be grown in liquid media. (b) Differentiated or stem/PCs retrieved from bronchial brushing, nasal or bronchoalveolar lavage (BAL). After induction, they may form spheroids in matrigel. Such spheroids can be generated through 3 methods: [1] After forming the 2D ALI cultures, spheroids will be formed from them; [2] By adding self-renewal cues; [3] The conditioned reprogramming culture (CRC) [136]