Fig. 6

GAA targeting LRPPRC inhibits cell proliferation, migration, and invasion in TNBC cells. A Cell proliferation curves of SUM-159 cells (left) and MDA-MB-468 cells (right) treated with GAA for 120 h. Two-way ANOVA test was used. B Representative colony formation images of SUM-159 and MDA-MB-468 cells after being treated by GAA. Colony formation data were summarized from independent experiments in triplicates and shown in histograms. One-way ANOVA test was used. C Transwell assays to detect cell migration of SUM-159 and MDA-MB-468 cell lines treated with GAA. Histogram analyses of migrated cell counts are shown. The number of migration cells was counted in ten random fields under a microscope. One-way ANOVA test was used. D Transwell assays to detect cell invasion of SUM-159 and MDA-MB-468 cell lines treated with GAA. Histogram analyses of migrated cell counts are shown. The invasive cells were counted in ten random fields under a microscope. One-way ANOVA test was used. E Cell proliferation of SUM-159 cells (before and after LRPPRC knockdown) treated with GAA 120 h. Unpaired t-test was used. F Quantification of normalized cell index of SUM-159 and MDA-MB-468 cells treated with GAA for 120 h