Fig. 5

GAA inhibits the synthesis of the OXPHOS complex. A Quantification of the mRNA of complex I/III, and complex IV/V mRNA in SUM-159 cells treated with GAA. The components of complex II are all encoded by the nuclear genome and are not shown in the figure. One-way ANOVA test was used. B Quantification of the mRNA of complex I/III, and complex IV/V mRNA in MDA-MB-468 cell treated with GAA. The components of complex II are all encoded by the nuclear genome and are not shown in the figure. One-way ANOVA test was used. C Immunoblotting of OXPHOS complex subunits in SUM-159 and MDA-MB-468 cells treated with GAA. D OCR analysis of SUM 159 cells treated with GAA. E OCR analysis of MDA-MB-468 cells treated with GAA. F Quantification of basal ORC of SUM-159 and MDA-MB-468 cells treated with GAA. One-way ANOVA test was used. G Quantification of MT ATP of SUM-159 and MDA-MB-468 cell lines treated with GAA. One-way ANOVA test was used