Fig. 3
LRPPRC promotes cell proliferation, migration, and invasion. A Immunoblotting of LRPPRC protein in SUM-159 and MDA-MB-468 cells treated with lentivirus containing control shRNA or LRPPRC-specific shRNA. B Representative colony formation images of SUM-159 and MDA-MB-468 cell lines treated with lentivirus containing control shRNA or LRPPRC-specific shRNA. Colony formation data were summarized from independent experiments in triplicates and shown in histograms. Unpaired t-test was used. C Cell proliferation curves of SUM-159 (top) and MDA-MB-468 cells (bottom) treated with lentivirus containing control shRNA or LRPPRC-specific shRNA. Two-way ANOVA test was used. D Transwell assays to detect cell migration of SUM-159 and MDA-MB-468 cell lines treated with lentivirus containing control shRNA or LRPPRC-specific shRNA. Histogram analyses of migrated cell counts are shown. The number of migration cells was counted in ten random fields under a microscope. The scale bar is 50 μm. Unpaired t-test was used. E Transwell assays to detect cell invasion of SUM-159 and MDA-MB-468 cell lines treated with lentivirus containing control shRNA or LRPPRC-specific shRNA. Histogram analyses of migrated cell counts are shown. The invasive cells were counted in ten random fields under a microscope. The scale bar is 50 μm. Unpaired t-test was used. Subcutaneous injection of mice with 1 × 107 LRPPRC-shRNA-1 SUM-159 cells and negative control SUM-159 cells. Tumors were dissected at the end of the experiment. F Representative IHC images and quantification of IHC intensity of LRPPRC protein in subcutaneous TNBC tissues. Data were summarized from 5 independent mice in each group and shown in the pie graph. G Tumor volume of subcutaneous TNBC tissues with postinjection time. Two-way ANOVA test was used. H Tumor weight of subcutaneous TNBC tissues. Unpaired t-test was used