Fig. 4

5hmC plays a crucial role in the differentiation of TET3-OE HUCMSCs to iOPCs. a, b Immunofluorescence staining for 5hmC in TET3-OE 7dpi and Control 7dpi (scale bar = 100 μm, n = 6 samples). Data were shown as mean ± standard deviation. Statistical significance was determined using unpaired t-test, ***p < 0.001.c Dynamic monitoring of 5hmC and 5mC changes during the differentiation process via dot blot. d, e Quantification of methylation levels in NG2 (d) and PDGFRA (e) promoters. Data were shown as mean ± standard deviation. Statistical significance was determined using unpaired t-test, ***p < 0.001. f Bisulfite Sequencing PCR analysis of NG2 and PDGFRA promoter regions