Fig. 5

TCDCA treatment alleviated severe acute pancreatitis in mice. a-g 3 mM TCDCA was added to the drinking water for 4 weeks before caerulein injection SAP models. Samples were at 12 h post caerulein-induced SAP modeling (n = 6). a Schematic illustration of experiments in complement of TCDCA and SAP models induced by caerulein injection. b Serum amylase activity and c serum lipase activity of SAP mice. n = 3 biologically independent samples. d Pathological sections and e pancreatic pathology scores from mice from a. Histopathological changes were scored by HE staining. Blue arrows indicate acinar necrosis, pink arrows indicate inflammatory cell infiltration, and black arrows indicate edema. n = 3 biologically independent samples. f Serum expression (ELISA) of IL-1β (left), IL-6 (mid) and TNF-α (right) in WT or Baat^−/− SAP mice. n = 3 biologically independent samples. g Relative mRNA expression of Il1b (left) and Il6 (right) in the pancreas in WT or Baat^−/− SAP mice. Fold change is relative to respective mock mice. n = 3 biologically independent samples. Data are presented as mean ± s.d. The two-sided P values were examined using Student’s t-test for comparison of variables between two groups. One-way ANOVA followed by Tukey’s multiple-comparison test was used for comparison of continuous variables among multiple groups. *: P < .05; **: P < .01; ***: P < .001; ****: P < .0001