Fig. 2

Acute pancreatitis was exacerbated in Baat^−/− mice. a Schematic illustration of experiments in SAP surgical models induced by 5% sodium taurocholate injection with species of WT and Baat^−/− mice. b Survival kinetics in surgical SAP model. Survival was observed for 72 h following retrograde injection of 5% sodium taurocholate into the pancreatic-bile duct in WT or Baat^−/− SAP mice (n = 25 individuals/group). c—(j) Samples were collected at 72 h post-retrograde injection in Surgical SAP model (n = 4—6). c Serum amylase (n = 6) and d serum lipase (n = 4) level of mice from a at 72 h post-retrograde injection with species of WT and Baat^−/− mice. n = 3 biologically independent samples. e Pancreatic pathological sections and f pancreatic pathology scores from a. Histopathological changes were scored by HE staining. The pathology was semi-quantitatively scored using Schmidt’s criteria by two board-certified veterinary pathologists in a double-blinded manner. The final pathology score is expressed as the average of these two values. Blue arrows indicate acinar necrosis, pink arrows indicate inflammatory cell infiltration, and black arrows indicate edema. n = 3 biologically independent samples. g Serum expression (ELISA) of IL-1β (left), IL-6 (mid) and TNF-α (right) in WT or Baat^−/− SAP mice. n = 3 biologically independent samples. Relative mRNA expression of Il1b (left), Il6 (mid), and Tnfa (right) in the h pancreas and i spleen of WT or Baat^−/− SAP mice (n = 6). Fold change is relative to respective mock mice. n = 3 biologically independent samples. j Infiltration of macrophages (CD11b⁺/F4/80⁺) in the pancreas of WT or Baat^−/− SAP mice shown via IFA. Results are representative of data generated in at least two independent experiments and are expressed as mean ± s.d. The two-sided P values were examined using Student’s t-test for comparison of variables between two groups. *: P < .05; **: P < .01; ***: P < .001; ****: P < .0001