Fig. 7

Both AKT and PKM2 inhibitors repress the role of SULT2B1 in regulating cell proliferation in vivo. A The schematic diagram of the animal experiment. B–D Xenograft mouse models in nude mice with OE-Ctrl and OE-SULT2B1 cells were constructed, and then treated with API-2 as well as Shikonin. The volume as well as weight of tumors were measured and visualized, and the photos of tumors in xenograft models were shown. E–G HE and IHC staining were conducted to analyze the cell proliferation in vivo as described above (scale bars = 50 μm and 6 μm). H The tumor tissues were grinded and lysed, and then the lysates were subjected to immunoblotting with indicated antibodies. *P < 0.05 versus control; **P < 0.01 versus control