Fig. 5

PKM2 combing with SULT2B1 contributes to the cell proliferation and migration regulated by SULT2B1. A Immunoprecipitation was performed with Flag and SULT2B1 targeted antibodies in OE-SULT2B1 and OE-Ctrl cells, respectively, and then the lysates were subjected to proteomic identification. B Co-immunoprecipitation was performed with Flag primary antibody in OE-SULT2B1 lysates. C Immunofluorescence co-localization was conducted with SULT2B1 and PKM2 primary antibody in OE-SULT2B1 cells. D The PKM2 expression was monitored in the indicated cells by immunoblotting. E–J Cell proliferation and migration abilities in the indicated cells were analyzed by MTS assay, wound healing assay (scale bars = 0.5 mm), colony growth assay (scale bars = 1 cm) and EDU staining assay (scale bars = 0.5 mm). The data were visualized by histogram graphs. K The P21 and P27 expressions were determined by immunoblotting in the indicated cells. *P < 0.05 versus control; **P < 0.01 versus control