Fig. 4

SULT2B1 positively promotes cell proliferation in vivo. A and C Xenografts of CRC cells in nude mice were constructed, and the tumor volumes were calculated and recorded at the indicated time points (The time of tumor excision from euthanized mice, A: 25 day; C: 20 day). The data were analyzed and visualized by the Prism GraphPad software. B and D Tumor weights were recorded and shown. E and F The tumors were photographed and shown. G HE staining was performed to analyze cell proliferation in specific tumor tissues (scale bars = 150 μm). H–K IHC staining was conducted in indicated tumor tissues with SULT2B1, p-AKT, t-AKT and Ki67 primary antibodies and quantification analysis was performed (scale bars = 50 μm and 6 μm). L Immunoblotting was carried out to detect the expressions of indicated molecules in xenografts tumors. *P < 0.05 versus control; **P < 0.01 versus control