Fig. 2

SULT2B1 promotes cell proliferation and migration in CRC cells. A KO-SULT2B1 HT29 cells and OE-SULT2B1 SW480 cells were constructed as described in methods, and efficacies were confirmed by immunoblotting. B Cell viability was detected by MTS assay. C Colony growth assay was conducted to monitor cell proliferation ability (scale bars = 1 cm). D Wound healing test was performed in the indicated cells, and pictures were taken at 0 h as well as 24 h time points (scale bars = 0.5 mm). E Transwell assay was carried out to monitor cell invasion (scale bars = 0.5 mm). F Indicated cells were collected and stained, and then flow cytometry was performed to analyze cell cycle. G EDU staining assay was conducted to detect cell proliferation (scale bars = 0.5 mm). H and I The lysates were prepared from indicated cells, and analyzed by immunoblotting with indicated antibodies. *P < 0.05 versus control; **P < 0.01 versus control