Fig. 6

MCL regulates PFKFB3-driven glycolysis. A Volcano plot showing PFKFB3 downregulation after MCL pretreatment (LPS + IFNγ + MCL group vs LPS + IFNγ group). B Relative mRNA expression of PFKFB3. C WB was used to quantify the protein levels of partial glycolysis-related proteins. D Levels of partial respiratory chain proteins were measured using WB. E Lactic acid levels in BMDM supernatants. F ATP levels. G iNOS and IL-1β expression after PFKFB3 overexpression. H IL-1β, IL-6, and TNF-α mRNA expression following PFKFB3 overexpression. I Mitochondrial membrane potential was estimated using the JC-1 kit (scale bar = 10 μm). J Mitochondrial ROS production was measured by MitoSOX™ Red (scale bar = 10 μm). K Change in the NADPH/NADP⁺ ratio after PFKFB3 overexpression. L PFKFB3 expression level after NAC pretreatment. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 3)