Fig. 2

MCL ameliorates M1-type macrophage polarization and the inflammatory response induced by LPS and IFN-γ. A MCL structure. B, C RAW264.7 cells and BMDMs were exposed to MCL for 24 h, and cell viability was determined by CCK8. D Protein expression of IL-1β, IL-6, and TNF-α in RAW264.7 cells. E Protein expression of IL-1β, IL-6, and TNF-α in BMDMs. F Levels of IL-1β, IL-6, and TNF-α in the supernatants of RAW264.7 cells (MCL: 10 μM). G Levels of IL-1β, IL-6, and TNF-α in the supernatants of BMDMs (MCL: 5 μM). H Protein expression of iNOS in RAW264.7 cells. I Protein expression of iNOS in BMDMs. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = not significant, compared with the IFNγ + LPS group (n = 3)