Fig. 5
Effect of 2-APB on light-induced action potential generation highlights the role of SACNS. Representative action potentials recorded from Ziapin2-loaded cells before and after treatment with 2-Aminoethyl Diphenylborinate 75 µM (2-APB) and stimulated with 20 ms- (panel a) or 200 ms-long (panel b) single light pulses. Traces have been reported as relative Vm variation to better appreciate the light-induced effect; photoexcitation is represented by the cyan shaded area. Light power density was set at 50 mW/mm2. c) Percentage of light-induced APs in Ziapin2-loaded AMVMs before and after treatment with 2-APB. d) Scatter plot of the peak hyperpolarization (ΔVm hyp Ctrl 20 ms: -2.68 ± 0.31 mV, n = 11; ΔVm hyp 2-APB 20 ms: -2.62 ± 0.19 mV, n = 12; ΔVm hyp Ctrl 200 ms: -2.57 ± 0.4 mV, n = 10; ΔVm hyp 2-APB 200 ms: -1.74 ± 0.12 mV, n = 13) and time-to-peak of hyperpolarization (tpeak hyp Ctrl 20 ms: 15.9 ± 1.2 ms, n = 20; tpeak hyp 2-APB 20 ms: 14.6 ± 1.5 ms, n = 11; tpeak hyp Ctrl 200 ms: 25.6 ± 4.1 ms, n = 12; tpeak hyp 2-APB 200 ms: 20.3 ± 2.9 ms, n = 10) in AMVMs exposed to 25 µM Ziapin2 for the above-mentioned light-stimulation protocols. Data are represented as mean ± SEM; Ctrl: N = 4. Statistical comparisons were performed using the Fisher’s exact test (panel c) and the ordinary one-way ANOVA test (panel d)