Fig. 3
HCN channel blockade by Ivabradine does not affect light-induced action potentials in AMVMs. Representative action potentials recorded in Ziapin2-loaded cells treated with Ivabradine 10 µM (IVA) and stimulated with 20 ms- (panel a) or 200 ms-long (panel b) single light pulses. Photoexcitation is represented by the cyan shaded area. Light power density was set at 50 mW/mm2. c) Percentage of light-induced APs in Ziapin2-loaded AMVMs before and after treatment with IVA. d) Scatter plot of the peak hyperpolarization (ΔVm hyp Ctrl 20 ms: -2.62 ± 0.54 mV, n = 8; ΔVm hyp IVA 20 ms: -1.74 ± 0.21 mV, n = 8; ΔVm hyp Ctrl 200 ms: -1.7 ± 0.37 mV, n = 8; ΔVm hyp IVA 200 ms: -1.63 ± 0.28 mV, n = 7) and time-to-peak of hyperpolarization (tpeak hyp Ctrl 20 ms: 19.7 ± 0.79 ms, n = 8; tpeak hyp IVA 20 ms: 21.1 ± 1.2 ms, n = 8; tpeak hyp Ctrl 200 ms: 19.7 ± 1.17 ms, n = 8; tpeak hyp IVA 200 ms: 28.79 ± 3 ms, n = 7) in AMVMs exposed to 25 µM Ziapin2 for the above-mentioned light-stimulation protocols. Data are represented as mean ± SEM; N = 2. Statistical comparisons were performed using the chi-square test (panel c) and the Kruskal-Wallis test (panel d)