Fig. 4

Knockdown of RBIS inhibits in vivo tumor growth and metastasis of lung cancer cells. A–C A549 cells stably expressing shNC or shRBIS were injected subcutaneously into 6-week-old BALB/c female nude mice (n = 20). The tumor images (A), tumor weights (B), and tumor volumes (C) are presented 31 days postinjection. D–F A549 cells stably expressing shNC or shRBIS were injected into the tail vein of 6-week-old male NOD-SCID mice (n = 6). Representative images of lung metastasis (D) and the incidence of lung metastasis (E) and H&E (F) results are shown. The Mann–Whitney test was used to assess differences in tumor weight and tumor volume among the different experimental treatment groups. The Chi-square test was used to evaluate the incidence of lung metastasis.  All experiments were conducted with three replicates