Fig. 6

TLR2 blockade attenuated the effects of LPS. A. The mRNA and protein expression of IL-6 after blocking TLR2 (5 µg/ml). IL-6 mRNA expression: Ctrl vs. LPS vs. LPS + IgA vs. IgA = 1.0 ± 0.3 vs. 9.0 ± 1.3 vs. 4.8 ± 0.4 vs. 1.0 ± 0.1, P < 0.001; IL-6 protein expression: Ctrl vs. LPS vs. LPS + IgA vs. IgA = 62.4 ± 11.8 pg/ml vs. 210.6 ± 14.7 pg/ml vs. 61.1 ± 30.5 pg/ml vs. 49.9 ± 12.4 pg/ml, P < 0.001. B. The mRNA and protein expression of claudin-1 after blocking TLR2 (5 µg/ml). Claudin-1 mRNA expression: Ctrl vs. LPS vs. LPS + IgA vs. IgA = 1.0 ± 0.0 vs. 0.8 ± 0.0 vs. 0.9 ± 0.0 vs. 0.9 ± 0.0, P < 0.001; claudin-1 protein expression: Ctrl vs. LPS vs. LPS + IgA vs. IgA = 1.0 ± 0.1 vs. 0.6 ± 0.1 vs. 1.0 ± 0.1 vs. 0.9 ± 0.1, P < 0.001. C. The intercellular space shown by H&E staining after blocking TLR2 (5 µg/ml). Ctrl vs. LPS vs. LPS + IgA vs. IgA = 0.4 ± 0.2 μm vs. 1.7 ± 0.5 μm vs. 0.5 ± 0.2 μm vs. 0.6 ± 0.2 μm, P < 0.001. Abbreviation: Ctrl: control; LPS: lipopolysaccharide; TLR: toll-like receptor; ***: P < 0.001