Fig. 6

MiR-195-5p regulates ECM and myofibroblast transdifferentiation of TGF-β-treated ESCs by targeting the YAP-Smad7 signaling pathway. A COIP detection of the targeting relationship between YAP and Smad7. B–F RT-PCR and Western blot analysis of YAP and Smad7 expression in uterine tissue after miR-195-5p mimic treatment. G–I Immunofluorescence analysis of YAP and Smad7 expression in uterine tissue after miR-195-5p mimic treatment. J–M Western blot analysis of YAP and Smad7 expression in uterine tissue after miR-195-5p mimic treatment and Smad7 knockdown. (ns = not significant, *P < 0.05, **P < 0.01, ****P < 0.001)