Fig. 1

MiR-195-5p and its downstream target gene Smad7 are decreased in IUA. A, B Heat map and volcano plot showing differential miRNAs in exosomes from normal and IUA groups. C Stem-loop RT-PCR results show significant decreases in miR-195-5p, miR-378a-5p, miR-30b-5p, and miR-30a-5p in SD rats with IUAs. D Differential expression of miR-195-5p in the endometrium of IUA patients. E Venn diagram of downstream target genes of human and rat miR-195-5p from miRDB and miRWalk databases. F Venn diagram showing the intersection of differential genes in the IUA transcriptome and miR-195-5p target genes. G Cytoscape shows the core genes of intersection genes. H Dual luciferase assays verify the targeting relationship between miR-195-5p and YAP, Smad7. I–K Western blot analysis of YAP and Smad7 expression in uterine adhesion tissues. (ns = not significant, *P < 0.05, **P < 0.01, ****P < 0.001)