Fig. 1

YAP1 is activated in HFD-fed mice and MAFLD patients. A Representative micrographs of immunofluorescence staining for pYAP1 in the crypt region of the ileum tissue from humans with and without MAFLD. Scale bars: 100 μm. B Quantitative analysis of A (n = 6 or 3). C, D Representative micrographs of immunohistochemistry results for nuclear pYAP1⁺ cells in the ileal crypts of the NonMAFLD and MAFLD patients and the quantitative analysis (n = 3). The arrows point to nuclear pYAP1⁺ cells. Scale bars: 25 μm. E mRNA expression of CTGF and CYR61 in the ileum tissues of humans with and without MAFLD (n = 6 or 3). F Representative micrographs of immunofluorescence staining for pYAP1 in the crypt region of the ileum tissue in mice. Scale bars: 50 μm. G Quantitative analysis of the data in F (n = 7). H, I Representative micrographs of immunohistochemistry results for nuclear pYAP1⁺ cells in the ileal crypts of the LFD- and HFD-fed mice and the quantitative analysis (n = 6). The arrows point to nuclei of pYAP1⁺ cells. Scale bars: 25 μm. J mRNA expression of Ctgf and Cyr61 in mouse ileum tissue (n = 5). LFD, low-fat diet; HFD, high-fat diet; pYAP1, phosphorylated YAP1; NonMAFLD, without MAFLD. *P < 0.05, **P < 0.01, ***P < 0.001