Fig. 4

HAS-CIRCpedia-5280 inhibits colon cancer cell proliferation and migration and promotes autophagy via hBD-1. (A): RT-qPCR detected has-circpedia-5280 and miR-4712-5p expression. (B): CCK-8 cell proliferation curve. (C): The cell migration ability was detected using the scratch assay. (D): MDC staining for intracellular autophagic vesicles. (E): Western blotting analysis for detecting the AKT/mTOR signaling pathway related proteins, LC3II/I ratio, and Beclin-1 expres-sion, the representative Western blotting bands, and the quantification of protein expression. Versus. oe-NC, *p<0.05, **p<0.01; Versus. oe-NC+hBD-1, #p<0.05, ##p<0.01; Versus. oe-circRNA, $p<0.05, $$p<0.01