Fig. 1

HBD-1 inhibited the proliferation of colon cancer cells and promoted autophagy. (A, B): CCK-8 was used to detect the cell proliferation curve. (C): The c ell migration ability was detected using the scratch assay. (D): MDC staining was utilized to assess the presence of autophagic vesicles in the cells. (E): Western blotting analysis was performed to detect the AKT/mTOR signaling pathway-related proteins, LC3II/I ratio, and Beclin-1 expression. The representative Western blotting bands and the quantification of protein expression are shown. Statistical significance was denoted as the control group. hBD-1: Human beta-defensin-1,*p<0.05, **p<0.01