Fig. 5

Suppression of inflammation in the AR mouse model by knocking down LRRC8A. A Schematic diagram of AR models. B, C Western blotting was used to detect LRRC8A protein expression in different groups of AR mouse models. D, E Comparison of sneezing and nasal rubbing frequencies within a 10-min period among different groups of mice. F–H qRT-PCR analysis of mRNA levels of Th2 cytokines IL-4, IL-5, and IL-13 in mouse nasal mucosa tissues. I–L ELISA was used to detect levels of OVA-specific IgE in serum and IL-4, IL-5, and IL-13 in supernatants of splenocytes in different groups. M, N HE staining of mouse nasal mucosa sections to monitor eosinophil infiltration (scale bar = 20 μm), with arrows indicating typical eosinophils. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001