Fig. 3

Knockout of LRRC8A improves mitochondrial oxidative stress and function in HNEpCs, and activates the NF-κB pathway. A Representative fluorescence co-localization images of NOX4 and Mito Tracker Red in HNEpCs transfected with siLRRC8A and NCsirna before IL-13 stimulation (scale bar = 10 μm). B Fluorescence intensity curves of NOX4 (Green) and Mito Tracker Red (Red) co-localization images in treated HNEpCs. C, D Mitosox staining of HNEPCs post-IL-13 stimulation reveals mitochondrial ROS expression (scale bar = 20 μm). E, F Changes in mitochondrial membrane potential were assessed using TMRM staining (scale bar = 50 μm). G Detection of mitochondrial DNA copy number levels using a mitochondrial mtDNA copy number assay kit. H Measurement of Mn-SOD enzyme activity expression levels. I–K Before IL-13 stimulation, HNEpCs were treated with siLRRC8A or NCsirna, followed by Western blotting to assess NF-κB p-65 and P-p65 protein expression. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001