Fig. 1
From: LRRC8A drives NADPH oxidase-mediated mitochondrial dysfunction and inflammation in allergic rhinitis
LRRC8A mRNA expression correlates with NADPH oxidase subunits and clinical characteristics, with LRRC8A, NOX1, NOX4, and p22phox upregulated in the nasal mucosa of allergic rhinitis patients compared to controls. A–G qRT-PCR was used to detect the mRNA expression levels of LRRC8A, NOX1, NOX4, p22phox, IL-4, IL-5, and IL-13 in human nasal mucosa (Con n = 18, AR n = 30). H–O Correlation analysis was conducted between LRRC8A mRNA and the mRNA levels of NOX1, NOX4, p22phox, IL-4, IL-5, IL-13(Con n = 18, AR n = 30), serum total IgE(Con n = 15, AR n = 26), and serum Phadiatop Test-specific IgE (Con n = 15, AR n = 24). P Protein expression of LRRC8A, NOX1, NOX4, and p22phox in human nasal mucosal homogenates as detected by Western blotting (Con n = 5, AR n = 5). Q Immunohistochemical analysis showing the expression of LRRC8A, NOX1, and NOX4 in the epithelial layer(scale bar: 20 μm). The circled regions highlight the areas where these proteins are expressed (Con n = 8, AR n = 8). Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001