Fig. 3

MST1 Overexpression aggravates neuronal damage in 5-month 5xFAD mice. A Representative Nissl staining images of hippocampal three subregions (DG, CA1 and CA3) in different group (Scale bar is 200 µm and enlarged images scale bar is 50 µm). red arrows indicate dead neuronal cells. B The ratio of live and dead neuronal cells of the hippocampus in different groups (n = 4). C HE staining images of hippocampal three subregions (DG, CA1 and CA3) in different group (n = 3, Scale bar is 200 µm and enlarged images scale bar is 50 µm). D Representative immunoblotting bands of hippocampus Bax, Bcl-2, Cleaved Caspase 9, Cleaved Caspase 3, and Cyt-C after AAVs injection E Quantitative analysis of Bax/Bcl-2, Cleaved Caspase 9, Cleaved Caspase 3, and Cyt-C in hippocampus (n = 4). F–H Representative immunofluorescence images and quantitative analysis of PSD95 and SYP in the hippocampus DG region of different group (n = 4, Scale bar is 50 µm). Nuclei were stained in blue (DAPI). I The levels of Aβ1-42 in the hippocampus of different group were measured using ELISA (n = 4). All data represent means ± SEMs. *, Comparison between C57 + AAV-vehicle and 5xFAD + AAV-vehicle group, *p < 0.05; **p < 0.01; ***p < 0.001; #, Comparison between 5xFAD + AAV-vehicle and 5xFAD + AAV-MST1 group, #p < 0.05; ##p < 0.01. ns, no significance